The HPLC Survival Guide

When your HPLC method goes wrong, know where to look next.A blank shows a peak. Pressure suddenly rises. Your peaks start tailing. Two compounds co-elute. Retention time drifts. Your gradient behaves differently than expected.What do you check first?The HPLC Survival Guide: The Lab Detective's Handbook is a practical field manual for diagnosing and troubleshooting real-world reversed-phase HPLC problems in pharmaceutical QC and method development. Instead of academic theory alone, it uses a problem-first, diagnostic approach: recognise the symptom, investigate the likely causes, and decide the right next action.14 field missions, including: - Ghost peaks, baseline noise and backpressure- Peak shape problems, stationary-phase selection and mobile-phase control- First-run method development and retention-time drift- Resolution, co-elution and gradient problems- UV/DAD detection, calibration and system suitability- Peak integration, sample and injection effects- Method transfer and robustnessPlus practical reference material, troubleshooting tools and checklists for interpreting chromatographic behaviour.Who it's for: QC analysts, method development scientists, lab technicians and supervisors - anyone who needs to move from "What happened?" to "What should I check next?"Stop guessing. Start diagnosing.Part of the Practical Pharma Science(TM) series - applied knowledge for pharma professionals.- Mirela Gorjanu, Practical Pharma Science(TM)